9NKY

Crystal structure of unliganded Fab MAM01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-18
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	52.766, 69.789, 115.054
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.110 - 2.080
R-factor	0.2371
Rwork	0.233
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.677
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.090	0.430
Rpim	0.047	0.202
Number of reflections	24638	1282
<I/σ(I)>	33.2
Completeness [%]	94.1
Redundancy	5.3
CC(1/2)	0.997	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M MES pH 6.0 and 30% (w/v) polyethylene glycol 6000