9NKT

[0,8,12P-->13] Shifted tensegrity triangle with an (arm,center,arm) distribution of (0,8,12) base pairs, 1 nt sticky ends, and 5' phosphates containing a semi-junction that becomes left-handed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	114.348, 114.348, 68.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.010 - 6.420
R-factor	0.3237
Rwork	0.314
R-free	0.39820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.749
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.174	57.174	7.695
High resolution limit [Å]	6.420	9.723	6.424
Number of reflections	599	199	203
<I/σ(I)>	3.4
Completeness [%]	87.3
Redundancy	8.3
CC(1/2)	0.976	0.975	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate