9NKA

[-1,8,12-->14] Shifted tensegrity triangle with an (arm,center,arm) distribution of (-1,8,12) base pairs and 2 nt sticky ends containing a semi-junction that becomes left-handed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000010
Spacegroup name	H 3
Unit cell lengths	112.173, 112.173, 67.872
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.380 - 4.880
R-factor	0.1879
Rwork	0.186
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.682
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.087	56.086	5.353
High resolution limit [Å]	4.877	9.725	4.877
Number of reflections	1316	186	189
<I/σ(I)>	9.4
Completeness [%]	89.3
Redundancy	9.1
CC(1/2)	0.999	0.999	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate