9NJZ

[0,8,12-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (0,8,12) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	100.621, 100.621, 110.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.740 - 4.180
R-factor	0.1729
Rwork	0.173
R-free	0.17510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.864
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.359	68.359	4.484
High resolution limit [Å]	4.180	10.278	4.185
Number of reflections	2648	205	204
<I/σ(I)>	10.3
Completeness [%]	89.9
Redundancy	8.9
CC(1/2)	0.999	0.999	0.144

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate