9NJH

Translocated product complex of DNA polymerase iota with DNA (template A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-08
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9686
Spacegroup name	P 65 2 2
Unit cell lengths	97.926, 97.926, 194.974
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.960 - 2.290
R-factor	0.2653
Rwork	0.264
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.636
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.960	2.370
High resolution limit [Å]	2.290	2.290
Rpim	0.024	0.399
Number of reflections	25733	2462
<I/σ(I)>	26.9	1.8
Completeness [%]	100.0	100
Redundancy	38.7	39.2
CC(1/2)	1.000	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	100 mM HEPES pH 7 1.5 -1.8 M Ammonium Sulfate 5 mM EDTA