9NJ1

GlfT2 from Nocardia brasiliensis Bound to Galf Tetrasaccharide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	106.210, 203.912, 292.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.880 - 3.150
R-factor	0.2388
Rwork	0.238
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	0.608
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.880	34.880	3.260
High resolution limit [Å]	3.150	6.770	3.150
Rmerge	0.356	0.094	1.521
Number of reflections	108981	11132	10701
<I/σ(I)>	5.1
Completeness [%]	98.6
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	6 mg/mL protein, 0.2% Triton X-100, 0.066 M imidazole, 2 mM magnesium chloride, 8.75% w/v PEG1000, 8.75% w/v PEG3350, 8.75% v/v MPD