9NJ0

GlfT2 from Nocardia brasiliensis Bound to Galf Trisaccharide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	106.784, 204.965, 294.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.610 - 3.200
R-factor	0.2006
Rwork	0.200
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	XDS (20230630)
Data scaling software	XDS (20230630)
Phasing software	PHENIX (1.21.2_5419)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.610	34.610	3.310
High resolution limit [Å]	3.200	6.880	3.200
Rpim	0.135	0.038	0.435
Number of reflections	101657	10321	10021
<I/σ(I)>	5.5	14.6	1.6
Completeness [%]	94.9	92.27	94.73
Redundancy	5.2	5.47	5.27
CC(1/2)		0.079	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	6 mg/mL protein, 0.2% Triton X-100, 0.066 M imidazole, 2 mM magnesium chloride, 8.75% w/v PEG1000, 8.75% w/v PEG3350, 8.75% v/v MPD