9NIB

Black sea bass polyomavirus LTA NLS bound to improtin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.802, 89.188, 99.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 2.200
R-factor	0.1909
Rwork	0.190
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.064	0.758
Rmeas	0.073	0.856
Rpim	0.033	0.385
Number of reflections	34155	2994
<I/σ(I)>	11.4	2.2
Completeness [%]	94.9	96.4
Redundancy	4.2	4.3
CC(1/2)	0.998	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.65 M Na citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0