9NI2
Fab369 in complex with the C-terminal alpha-TSR domain of the P. falciparum circumsporozoite protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977410 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.294, 71.098, 101.969 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 49.260 - 2.130 |
| R-factor | 0.1894 |
| Rwork | 0.187 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5127: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.720 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.207 | 1.526 |
| Rmeas | 0.225 | 1.705 |
| Rpim | 0.087 | 1.000 |
| Number of reflections | 30592 | 2456 |
| <I/σ(I)> | 8.2 | 1 |
| Completeness [%] | 99.7 | 96.8 |
| Redundancy | 6.6 | 5 |
| CC(1/2) | 0.993 | 0.322 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.17 M ammonium sulfate, 15% glycerol, 25.5% PEG 4000 |
