9NHY
Fab1502 in complex with the C-terminal alpha-TSR domain of the P. falciparum circumsporozoite protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | I 41 |
| Unit cell lengths | 102.986, 102.986, 172.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.500 - 2.000 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.095 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.146 | 3.876 |
| Rmeas | 0.152 | 4.023 |
| Rpim | 0.041 | 1.070 |
| Number of reflections | 59911 | 2876 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 98.9 | 94.9 |
| Redundancy | 13.3 | 13.5 |
| CC(1/2) | 0.998 | 0.359 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M sodium citrate, 30% v/v PEG 400, 0.1 M Tris pH 8.5 |
