9NHF
The structure of a family 168 glycoside hydrolase from the marine bacterium Muricauda eckloniae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 47.274, 120.934, 163.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.650 - 2.000 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.463 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.310 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.132 | 0.978 |
| Rmeas | 0.142 | |
| Rpim | 0.051 | 0.364 |
| Number of reflections | 64544 | 4512 |
| <I/σ(I)> | 12.8 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 8 |
| CC(1/2) | 0.997 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 20% w/v PEG 3350, 8% v/v Tacsimate pH 7.0 |
