9NH9
Crystal Structure of N-oxygenase HrmI with the diferric cofactor and the N(6)-hydroxy-L-lysine product bound
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-22 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.520, 112.520, 67.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.500 - 1.960 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.905 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.760 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 35485 | 3508 |
| <I/σ(I)> | 8.95 | 1.79 |
| Completeness [%] | 99.6 | 99.91 |
| Redundancy | 10.3 | 10.3 |
| CC(1/2) | 0.996 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate and HEPES pH 7.5 |
