9NGT
Crystal structure of CRBN-DDB1 and FPFT-2216 in complex with mTOR
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 183.176, 81.213, 99.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 2.950 |
| R-factor | 0.2329 |
| Rwork | 0.231 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | DIALS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_5430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.240 | 3.050 |
| High resolution limit [Å] | 2.610 | 2.950 |
| Rmerge | 0.153 | |
| Number of reflections | 30713 | 1372 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 96.5 | |
| Redundancy | 11 | |
| CC(1/2) | 0.997 | 0.438 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 70 mM MES pH 6.0, 1.8-3.2 % (w/v) PEG 3,000, and 13-24 % (w/v) PEG 200 |
