9NG1
Crystal structure of FabG4 from Pseudomonas putida KT2440
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2023-05-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.724, 114.181, 144.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.740 - 1.910 |
| R-factor | 0.1727 |
| Rwork | 0.172 |
| R-free | 0.19858 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.740 | 1.644 |
| High resolution limit [Å] | 1.600 | 1.602 |
| Rmerge | 0.280 | 0.530 |
| Rpim | 0.144 | |
| Number of reflections | 113406 | 2159 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 87.2 | 24.53 |
| Redundancy | 8.4 | 1.7 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 Tris-HCl, 0.12 M ammonium chloride, pH 8.5, 20% PEG-4000 |
