9NFO

[6,7,7-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (6,7,7) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	107.460, 107.460, 90.958
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.810 - 4.030
R-factor	0.1942
Rwork	0.193
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.965
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.049	65.049	4.372
High resolution limit [Å]	4.026	10.356	4.026
Number of reflections	2319	193	192
<I/σ(I)>	9
Completeness [%]	88.1
Redundancy	10.7
CC(1/2)	0.998	0.998	0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate