9NFG

[4,8,8-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,8,8) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	135.133, 135.133, 42.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.010 - 5.770
R-factor	0.1912
Rwork	0.187
R-free	0.28750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.953
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.567	67.566	6.681
High resolution limit [Å]	5.766	11.289	5.766
Number of reflections	964	193	194
<I/σ(I)>	9
Completeness [%]	85.8
Redundancy	18
CC(1/2)	0.998	0.998	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate