9NEX

[2,8,10-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (2,8,10) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	133.078, 133.078, 47.855
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.270 - 6.760
R-factor	0.1112
Rwork	0.108
R-free	0.14910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.539	66.539	7.806
High resolution limit [Å]	6.756	11.525	6.756
Number of reflections	792	198	199
<I/σ(I)>	11.3
Completeness [%]	89.5
Redundancy	18.7
CC(1/2)	0.999	0.999	0.216

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate