9NEV

Acanthamoeba Polyphaga Mimivirus L230

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	110
Detector technology	PIXEL
Collection date	2021-04-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	109.610, 109.610, 84.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.015 - 2.300
Rwork	0.205
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.484
Data reduction software	xia2 (3.8.0)
Data scaling software	Aimless (0.7.8)
Phasing software	PHENIX (1.20.1)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.160	1.002
Number of reflections	23603	2273
<I/σ(I)>	15.7	4.2
Completeness [%]	100.0	100
Redundancy	25.7	27.1
CC(1/2)	0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein/cofactor mix: 15mg/ml L230, 500uM FeCl2 Mother Liquor: 0.04 M potassium dihydrogen phosphate, 16 % w/v PEG 8000, 20 % v/v Glycerol The ratio of mix and mother liquor was at 1:1.