9NE3

[1,8,10,P-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (1,8,10) base pairs, 1 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	133.933, 133.933, 49.824
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.700 - 6.890
R-factor	0.2076
Rwork	0.204
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.782
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.700	19.697	7.825
High resolution limit [Å]	6.890	10.960	6.895
Number of reflections	774	194	193
<I/σ(I)>	7.4
Completeness [%]	90.6
Redundancy	19.5
CC(1/2)	0.993	0.993	0.096

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate