9NDO

[2,8,9,P-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (2,8,9) base pairs, 2 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	134.129, 134.129, 43.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.530 - 6.800
R-factor	0.2141
Rwork	0.210
R-free	0.36620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.752
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.064	67.064	8.516
High resolution limit [Å]	6.798	10.862	6.798
Number of reflections	645	215	215
<I/σ(I)>	7.2
Completeness [%]	85.7
Redundancy	13.9
CC(1/2)	0.998	0.998	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate