9NDN

[0,8,11,P-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (0,8,11) base pairs, 2 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	133.505, 133.505, 49.719
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.700 - 6.410
R-factor	0.2232
Rwork	0.218
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.825
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.675	45.675	7.283
High resolution limit [Å]	6.410	11.885	6.413
Number of reflections	917	184	185
<I/σ(I)>	8.9
Completeness [%]	89.6
Redundancy	18.9
CC(1/2)	0.998	0.998	0.273

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate