9NDH

[4,8,8,P-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,8,8) base pairs, 1 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	131.404, 131.404, 49.315
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.850 - 5.780
R-factor	0.1601
Rwork	0.157
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.746
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.702	65.702	6.496
High resolution limit [Å]	5.777	11.559	5.777
Number of reflections	1178	196	200
<I/σ(I)>	14
Completeness [%]	89.2
Redundancy	17.7
CC(1/2)	1.000	1.000	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate