9NDE

[4,7,9P-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,7,9) base pairs, 1 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	106.135, 106.135, 91.129
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.460 - 4.110
R-factor	0.25
Rwork	0.248
R-free	0.27650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.917
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.714	64.714	4.498
High resolution limit [Å]	4.110	10.272	4.110
Number of reflections	2143	193	193
<I/σ(I)>	7.2
Completeness [%]	87.9
Redundancy	8.4
CC(1/2)	0.998	0.998	0.234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate