9NDC

[3,14,12-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (3,14,12) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000000
Spacegroup name	H 3
Unit cell lengths	167.842, 167.842, 92.111
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.930 - 6.340
R-factor	0.2393
Rwork	0.238
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.719
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.921	83.921	7.168
High resolution limit [Å]	6.340	14.062	6.341
Number of reflections	1323	189	189
<I/σ(I)>	7.5
Completeness [%]	83.3
Redundancy	9.5
CC(1/2)	0.995	0.995	0.154

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate