9ND2

[1,8,10-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (1,8,10) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000010
Spacegroup name	P 63
Unit cell lengths	133.130, 133.130, 49.937
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.650 - 6.610
R-factor	0.2366
Rwork	0.235
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.565	66.565	7.732
High resolution limit [Å]	6.587	11.511	6.587
Number of reflections	841	209	209
<I/σ(I)>	9.9
Completeness [%]	88.2
Redundancy	19
CC(1/2)	0.998	0.998	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate