9NCP

[3,8,8-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (3,8,8) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.007430
Spacegroup name	P 63
Unit cell lengths	131.716, 131.716, 45.774
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.930 - 5.590
R-factor	0.1996
Rwork	0.197
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.745
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.858	65.858	6.334
High resolution limit [Å]	5.586	11.275	5.586
Number of reflections	1161	194	193
<I/σ(I)>	9.8
Completeness [%]	87.3
Redundancy	16
CC(1/2)	0.998	0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate