9NCM

[4,7,9-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,7,9) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000010
Spacegroup name	H 3
Unit cell lengths	106.888, 106.888, 91.525
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.680 - 4.150
R-factor	0.1883
Rwork	0.188
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.785
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.083	65.083	4.549
High resolution limit [Å]	4.147	10.343	4.147
Number of reflections	2125	194	191
<I/σ(I)>	16.8
Completeness [%]	87.7
Redundancy	10.7
CC(1/2)	1.000	1.000	0.287

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate