9NCH

[6,7,6,P-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (6,7,6) base pairs, 2 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	107.925, 107.925, 89.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.860 - 4.000
R-factor	0.2712
Rwork	0.270
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.624	64.624	4.287
High resolution limit [Å]	4.000	10.198	4.000
Number of reflections	2398	201	200
<I/σ(I)>	5.6
Completeness [%]	89.7
Redundancy	9
CC(1/2)	0.999	0.999	0.256

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate