9NBV

[5,7,8-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (5,7,8) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.99187
Spacegroup name	H 3
Unit cell lengths	110.456, 110.456, 76.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.520 - 5.970
R-factor	0.2893
Rwork	0.285
R-free	0.38090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.631
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.748	59.748	7.610
High resolution limit [Å]	5.966	9.943	5.966
Number of reflections	567	187	189
<I/σ(I)>	4.9
Completeness [%]	80.7
Redundancy	10.8
CC(1/2)	0.996	0.996	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM MOPS, 1.25 M magnesium sulfate