9NBU

[7,7,5-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (7,7,5) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	H 3
Unit cell lengths	106.722, 106.722, 89.627
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.070 - 4.790
R-factor	0.2319
Rwork	0.229
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.871
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.342	64.342	5.543
High resolution limit [Å]	4.790	9.950	4.792
Number of reflections	1261	208	211
<I/σ(I)>	8.7
Completeness [%]	85.4
Redundancy	9.9
CC(1/2)	0.996	0.996	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate