9NAC

Structure of HPK1 with C4 bound at its active site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	91
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	112.632, 52.343, 104.411
Unit cell angles	90.00, 104.16, 90.00

Refinement procedure

Resolution	33.750 - 1.450
R-factor	0.178
Rwork	0.177
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.704
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.630	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.074	0.611
Rmeas	0.080	0.660
Rpim	0.029	0.248
Total number of observations	707146	30074
Number of reflections	98887	4357
<I/σ(I)>	13.4	3.6
Completeness [%]	94.9
Redundancy	7.2	6.9
CC(1/2)	0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	not provided by author