9N9U
Camel alphacoronavirus (229E-like) Nsp5 in complex with AVI8122
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2024-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.502, 121.453, 103.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 - 2.300 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.270 | |
| Number of reflections | 13544 | 17608 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 95.1 | 98.43 |
| Redundancy | 12.8 | |
| CC(1/2) | 0.997 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 291.15 | 0.06 M Citric acid, 0.04 M BIS-TRIS propane / pH 4.1 , 16% w/v Polyethylene glycol 3,350 |
