9N89

Crystal structure of 2A2 malarial antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2024-10-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 21 21 21
Unit cell lengths	59.974, 76.682, 91.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.510 - 1.200
R-factor	0.1864
Rwork	0.186
R-free	0.19410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.797
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.510	1.210
High resolution limit [Å]	1.200	1.200
Number of reflections	131804	8275
<I/σ(I)>	8.88
Completeness [%]	99.5
Redundancy	3.5
CC(1/2)	0.997	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	30% w/v PEG 4K