9N80

Crystal structure of human HRSP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.968600
Spacegroup name	P 21 3
Unit cell lengths	88.486, 88.486, 88.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 1.390
R-factor	0.1923
Rwork	0.191
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.771
Data reduction software	XDS (Jun 30, 2024; Fast_DP version 1.6.2)
Data scaling software	XDS (Jun 30, 2024; Fast_DP version 1.6.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	1.440
High resolution limit [Å]	1.390	1.390
Rmerge	0.044	0.620
Number of reflections	46625	4619
<I/σ(I)>	56.71	5.88
Completeness [%]	100.0	100
Redundancy	37.9	29.5
CC(1/2)	1.000	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293.15	0.2 M sodium thiocyanate pH 6.9, 18 % PEG 3350, 30 mM N1-methyladenosine