9N7O
Designed anti-OSM Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2024-09-12 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.080, 127.080, 68.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.770 - 1.480 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.930 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.182 | 1.858 |
| Rmeas | 0.186 | 1.904 |
| Rpim | 0.036 | 0.412 |
| Total number of observations | 2455205 | 97460 |
| Number of reflections | 94102 | 4610 |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 100.0 | |
| Redundancy | 26.1 | 21.1 |
| CC(1/2) | 0.999 | 0.306 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 0.1 M Bis-Tris propane, pH 9.0, 10% v/v PEG200, 18% w/v PEG8000 |
