9N7M
Designed anti-OSM Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2024-09-12 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 |
| Unit cell lengths | 116.836, 116.836, 101.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.310 - 2.150 |
| R-factor | 0.2113 |
| Rwork | 0.210 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.512 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.224 | 0.707 |
| Rmeas | 0.251 | 0.810 |
| Rpim | 0.111 | 0.390 |
| Total number of observations | 375920 | 19268 |
| Number of reflections | 74105 | 4549 |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.1 | 4.2 |
| CC(1/2) | 0.984 | 0.154 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M ammonium sulfate, 0.1 M MES, pH 6.5, 30% w/v PEG5000 MME |
