9N7K

Crystal structure of human anti-Pfs48/45 transmission-blocking antibody RUPA-71

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-11-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95357
Spacegroup name	P 21 21 21
Unit cell lengths	64.660, 74.550, 204.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.850 - 2.280
R-factor	0.1932
Rwork	0.191
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.991
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	2.360
High resolution limit [Å]	2.280	2.280
Rpim	0.099	0.562
Number of reflections	46105	4434
<I/σ(I)>	7.5	1.7
Completeness [%]	100.0	100
Redundancy	15.4	15.2
CC(1/2)	0.991	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	100	0.2 M Ammonium Sulfate, 0.1 M MES, 30 %w/v PEG MME 5K