9N6G

EGFR(T790M/V948R) in complex with LN2827

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97920
Spacegroup name	P 1 21 1
Unit cell lengths	71.468, 101.884, 87.726
Unit cell angles	90.00, 102.55, 90.00

Refinement procedure

Resolution	85.630 - 2.590
Rwork	0.203
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.630	2.670
High resolution limit [Å]	2.590	2.600
Rmerge	0.135	0.135
Number of reflections	37605	4243
<I/σ(I)>	9.96
Completeness [%]	98.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	Bis-Tris (100mM), 25% PEG-3350 and TCEP (5mM)