9N6A
GP23 C-termimal receptor binding domain from TM4 phage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2020-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 |
| Unit cell lengths | 125.727, 125.727, 97.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.320 - 2.910 |
| R-factor | 0.2327 |
| Rwork | 0.230 |
| R-free | 0.28020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.198 | 1.898 |
| Number of reflections | 12488 | 648 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 3.9 | 3.8 |
| CC(1/2) | 1.000 | 0.254 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.2 M Lithium Sulfate 0.1 M MES: NaOH, pH 6 20 % (v/v) 1,4-Butanediol |
