9N5F

RNA polymerase II elongation complex with 8-oxoG in syn-conformation with added AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	167.540, 223.101, 195.002
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	49.220 - 3.600
R-factor	0.2487
Rwork	0.248
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.627
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	3.670
High resolution limit [Å]	3.600	3.600
Rmerge	0.559	8.839
Rmeas	0.600	9.478
Rpim	0.216	3.413
Total number of observations	622175	34496
Number of reflections	81363	4489
<I/σ(I)>	4.4	0.4
Completeness [%]	99.9
Redundancy	7.6	7.7
CC(1/2)	0.984	0.236

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000