9N5D
RNA polymerase II elongation complex with 8-oxoG at +1 site, CMP added
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97648 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.899, 222.270, 193.145 |
| Unit cell angles | 90.00, 100.27, 90.00 |
Refinement procedure
| Resolution | 49.310 - 3.350 |
| R-factor | 0.2652 |
| Rwork | 0.265 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.666 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 3.380 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.505 | 4.841 |
| Rmeas | 0.541 | 5.184 |
| Rpim | 0.194 | 1.853 |
| Total number of observations | 778362 | 38789 |
| Number of reflections | 100590 | 4969 |
| <I/σ(I)> | 5.2 | 0.5 |
| Completeness [%] | 99.3 | |
| Redundancy | 7.7 | 7.8 |
| CC(1/2) | 0.983 | 0.237 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
