9N55

X-ray Crystallographic Structure of Lipid-bound Orf9b Homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2022-10-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	35.850, 64.230, 73.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.380 - 1.650
R-factor	0.2006
Rwork	0.198
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.686
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.079	1.435
Rmeas	0.095	1.710
Number of reflections	38516	1312
<I/σ(I)>	7.22	0.97
Completeness [%]	98.1	85.11
Redundancy	3.4	3.1
CC(1/2)	0.995	0.228

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG3350, PEG1K, MPD, Ethylene Glycol, Imidazole, MES