9N54

Bipartite p63 NLS in complex with Importin Alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953725
Spacegroup name	P 21 21 21
Unit cell lengths	78.397, 90.142, 100.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.750 - 2.200
R-factor	0.1841
Rwork	0.183
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.591
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.750	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.062	0.547
Rmeas	0.070	0.613
Rpim	0.031
Number of reflections	36733	3134
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.999	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.65 M sodium citrate, 0.1 M HEPES and 10 mM DTT