9N51
Crosslinked Crystal Structure of Human Mitochondrial Ketosynthase, OXSM, and Crosslinker-crypto Human Mitochondrial Acyl Carrier Protein, C8Cl-mACP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.000, 118.630, 132.510 |
| Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
| Resolution | 53.640 - 2.310 |
| Rwork | 0.245 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.640 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.283 | 3.257 |
| Rmeas | 0.322 | 3.695 |
| Rpim | 0.151 | 1.721 |
| Total number of observations | 22413 | |
| Number of reflections | 104006 | 5146 |
| <I/σ(I)> | 4.4 | 0.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.3 | 4.4 |
| CC(1/2) | 0.979 | 0.263 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M ammonium sulfate, 1 M lithium sulfate, and 0.1 M sodium citrate pH 5.6 |
