Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2013-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.215, 80.961, 65.955 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 34.110 - 1.850 |
| R-factor | 0.1783 |
| Rwork | 0.176 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.057 | 0.030 | 0.667 |
| Total number of observations | 203038 | ||
| Number of reflections | 52895 | 5425 | 5219 |
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 98.3 | 98.8 | 97.5 |
| Redundancy | 3.8 | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M Lithium Sulfate, 0.1 M Tris pH8.5, 30% PEG4000 |
