9N3U

Structure of glyoxal oxidase from Fusarium graminearum at 1.28 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95302
Spacegroup name	C 2 2 21
Unit cell lengths	86.000, 129.980, 134.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.660 - 1.280
R-factor	0.1401
Rwork	0.140
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.281
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	1.320
High resolution limit [Å]	1.280	1.280
Number of reflections	187197	15957
<I/σ(I)>	16.7	2.09
Completeness [%]	97.6	95.3
Redundancy	13.7
CC(1/2)	0.990	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	295.15	0.2M lithium sulfate monohydrate, 0.1M bis-tris, 29% PEG 3350