9N3S
Crystal structure of the fungal mannosyltransferase Och1 reveals active site primed for N-glycan binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 228.15 |
| Detector technology | CCD |
| Collection date | 2009-08-10 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.447, 57.090, 84.088 |
| Unit cell angles | 85.57, 75.31, 75.13 |
Refinement procedure
| Resolution | 24.460 - 2.010 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.936 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (DEV_4694) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.460 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 44906 | |
| <I/σ(I)> | 4.81 | 4.34 |
| Completeness [%] | 89.8 | 58.2 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295.15 | 0.1 Bis-TRIS pH 6.5, 25% PEG 3350, 30 mM NaF |
