9N3G
FtsY bound to cyclic peptide P3k
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953730 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.544, 61.822, 73.369 |
| Unit cell angles | 95.35, 102.19, 96.54 |
Refinement procedure
| Resolution | 49.350 - 2.630 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 2.790 |
| High resolution limit [Å] | 2.628 | 2.630 |
| Rmerge | 0.126 | 1.182 |
| Rmeas | 0.148 | |
| Rpim | 0.077 | 0.721 |
| Number of reflections | 16116 | 2365 |
| <I/σ(I)> | 7.15 | 0.89 |
| Completeness [%] | 96.5 | 93.77 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.995 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 278 | 2% v/v Tacsimate pH 8,0 0.1M Tris pH 8.5, 16% PEG3350 |
