9N3E
Crystal structure of Arabidopsis metacaspase 9 C147G at pH 5.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.627, 89.046, 64.693 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 29.840 - 1.550 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.449 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.770 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 93555 | 6273 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.63 | |
| CC(1/2) | 0.996 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 5000 MME, sodium acetate, ammonium sulfate |
