9N30

Crystal Structure of LM2379

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.99997
Spacegroup name	P 31 2 1
Unit cell lengths	57.745, 57.745, 226.127
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.010 - 2.200
R-factor	0.2308
Rwork	0.229
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.475
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.170	2.668
Number of reflections	21936	1358
<I/σ(I)>	5.5	0.3
Completeness [%]	100.0	100
Redundancy	16.3
CC(1/2)	0.997	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.05 M Calcium chloride, 0.1 M BisTris pH 6.5, 30%(v/v) PEG 550 MME